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  • 洁净手术室空气细菌浓度的检测需要哪些条件?
    来源:http://www.moptuan.com 浏览: 发布日期:2017-06-14
    1.采样条件
    1 sampling conditions
    净化空调系统在开启状态,细菌浓度宜在其他项目检测完毕,对全室表面进行常规消毒之后进行。采样点设置,当送风口集中布置时,应对手术区和周边区分别检测;当送风口分散布置时,全室统一检测。测点布置原则,在集中送风条件下,凡手术室均采用3点布样;I级手术室辅助区采用8点布样即每边内2点;Ⅱ级手术室辅助区6点布样即每长边内2点;Ⅲ级手术室辅助区4点布样。当送风口分散布置时,按全室统一布点检测,测点可均布,但不应布置在送风口正下方。
    Clean air conditioning system in the open state, the concentration of bacteria should be detected in other projects, the whole room surface after routine disinfection. When the air inlet is arranged centrally, the operation area and the surrounding area should be detected respectively. When the air inlet is dispersed, the whole room is unified. The principle of measuring point arrangement in centralized air condition, where all operation room with 3 point pattern; I operation room auxiliary area with 8 point pattern that each side in 2 points; 2 operation room auxiliary zone 6. Each long side pattern in 2 points; grade 4 operation room auxiliary area pattern. When the air outlet is arranged in a decentralized way, the whole room is uniformly distributed, and the measuring points can be evenly distributed, but should not be arranged below the air outlet.
    2.采样方法
    2 sampling method
    采用浮游法测定浮游菌浓度时。测点数应和被测区域的含尘浓度测点数相同,每次采样时间不应超过30 min。用沉降法测定沉降菌浓度时,测点数既要不少于被测区域含尘浓度测点数,又要满足规定的少培养皿数的要求。采样时必须有2次空白对照。第1次与用于检测的培养皿做对比试验,每批一个对照皿。第2次是在检测时,每室或每区1个对照皿。
    When the concentration of planktonic bacteria was determined by floatation method. The measured points should be the same as those of the measured area, and the sampling time should not exceed 30 min. When the sedimentation method was used to determine the concentration of settling bacteria, the measured points were not only less than the measured concentration of dust in the measured area, but also meet the requirements of the minimum number of dishes. Sampling must have 2 blank control. The first test was done with a Petri dish for each batch. The second time was in the detection of 1 controls per room or area.
    3.采样高度:
    3 sampling height:
    采样点可布置在地面上或不高于地面0.8m的任意高度上;在手术区检测时应无手术台;当手术台已固定时,检测高度应在台面之上0.25cm。
    Sampling points can be placed on the ground or not higher than the ground 0。8m arbitrary height in the operation area should be no operating table when the operating table has been fixed when the detection height should be above the table 0。25cm。
    4.采样注意事项
    4 sampling notes
    放置采样平板从总面积中靠里的房间开始布置。依次向外布放,每间房间也是从房间靠里的位置开始布置。后布置门附近的点;收取平板时顺序相反,从外边的房间开始收,每间房间从靠门的开始收,先布置的皿后收;在洁净房间内布点均匀;设阴性对照平板。注意层流洁净室应尽量避开送风口正下方,若有固定设备如手术床等,可放置在设备上。
    Place the sampling plate from the top area of the total area. Each room is placed in the center of the room. Near the last door arrangement; charge plate in reverse order, from the outside of the room began to close, each room began to collect from the most close to the door, the first layout of the dish and finally closed in the clean room is uniform;; negative control panel. Note the laminar flow clean room should be avoided just below the efficient delivery outlet, if there is a fixed equipment such as surgical bed, can be placed on the device.

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