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  • 实验室洁净系统是生物安全实验室必备
    来源:http://www.moptuan.com 浏览: 发布日期:2017-08-29
    洁净空气调节系统是生物安全实验室安全运行的关键。由于生物安全实验室内操作对象是危险性的微生物,对人体、动植物或环境具有危害,必须通过采用合理的空气调节系统,保持生物安全实验室的相对负压环境无论何时都不被破坏,以保证实验室内危险性微生物不能向外部环境中扩散。
    Clean air conditioning system is the key to the safe operation of biosafety laboratories. The biological safety laboratory operation object is hazardous to the human body, microorganisms, animals and plants or the environment is harmful, we must regulate the system through the use of reasonable air, maintain a relatively negative environment of biological safety laboratory whenever it is not destroyed, in order to ensure laboratory dangerous microorganisms can not spread to the external environment.
    超净工作台/生物安全柜
    Super clean bench / biosafety cabinet
    细胞培养对无菌环境要求很高,在细胞操作时,一般需要在100级空气质量条件下进行,因此超净工作台或生物安全柜就必须配备。
    Cell cultures are very demanding for sterile environments and are usually performed under 100 levels of air quality during cellular operations, so the ultra clean bench or biosafety cabinet must be equipped.
    CO2培养箱
    CO2 incubator
    重症监护室装修
    CO2培养箱是细胞培养的必备仪器,它为细胞提供了一个体外培养的舒适环境,如适宜的温度、湿度、酸碱度、O2浓度等。细胞在体外培养时很容易受到各种细菌、微生物的感染,因此,CO2培养箱的灭菌和抑菌能力就显得尤为重要。
    CO2 incubator is an essential instrument for cell culture. It provides a comfortable environment for cells in vitro, such as suitable temperature, humidity, pH, O2 concentration and so on. When cells are cultured in vitro, they are susceptible to infection by a variety of bacteria and microorganisms. Therefore, the ability of sterilization and bacteriostasis of CO2 incubator is particularly important.
    恒温水浴
    Thermostatic bath
    从液氮或超低温冰箱中取出细胞冻存管需要立即放于37℃水浴中快速复苏之后才传代培养,因此,恒温水浴也是细胞实验室必备仪器。
    The cell cryopreservation tube from liquid nitrogen or ultra low temperature refrigerator needs to be placed in the water bath at 37 DEG C for a quick recovery and then subcultured. Therefore, the constant temperature water bath is also a necessary instrument in the cell laboratory.
    超低温冰箱
    Ultra low temperature freezer
    细胞的冻存过程需要一系列程序降温,一般4℃下存放30 min,转放-20℃ 1 hour,再转入-70至-80℃过夜,之后才可以转移到液氮内(-196℃),这时就必须配备一台-86℃超低温冰箱。一般超低温冰箱保存细胞可达数月,对于不需要长期冻存的细胞,可暂时放于超低温冰箱保存。
    The cryopreservation process requires a series of programmed cooling cells, usually under 4 DEG C for 30 min, a -20 C 1 hour, and then transferred to the overnight -70 to -80 DEG C, then can be transferred to liquid nitrogen (-196 C), then it must be equipped with a -86 ultra low temperature refrigerator temperature. In the ultra low temperature freezer, cells can be preserved for several months. For cells that do not need long-term cryopreservation, they can be stored in a cryogenic refrigerator for the time being.
    本文由重症监护室装修整理发布,您是不是对这些内容有所了解了呢?更多的内容请点击: http://www.moptuan.com我们会有更多精彩内容等你查看。
    This article is arranged by the intensive care unit. Do you know something about it? Please click on http://www.moptuan.com for more content. We will have more wonderful content for you to check.

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